Elevated Background in Double Antibody Sandwich-Indirect Enzyme-Linked Immunosorbent Assay for the Detection of Citrus tristeza virus in Mandarin Varieties
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Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
متن کاملThe Use of Enzyme - Linked Immunosorbent Assay for Detection of Citrus Tristeza Virus
BAR-JOSEPH, M., S. M. GARNSEY, D. GONSALVES, M. MOSCOVITZ, D. E. PURCIFULL, M. F. CLARK, and G. LOEBENSTEIN. 1979. The use of enzyme-linked immunosorbent assay for detection of citrus tristeza virus. Phytopathology 69:190-194. An enzyme-linked immunosorbent assay (ELISA) test was used to seedling yellows isolates of CTV from Israel and from Florida. Isolates that identify citrus tristeza virus ...
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ژورنال
عنوان ژورنال: International Organization of Citrus Virologists Conference Proceedings (1957-2010)
سال: 2010
ISSN: 0074-7203,2313-5123
DOI: 10.5070/c59j34h6c1